Some time ago I wrote about how to fit a linear model and interpret its summary table in R. At the time I used an example in which the response variable depended on two explanatory variables and on their interaction. It was a rather specific article, in which I overlooked some essential steps in the process of selection and interpretation of a statistical model. In the present article and in the relative worked examples I will expand the topic a bit, explaining 1) how to select the most parsimonious model relatively to a dataset using function *anova()* and 2) how to use *summary()*, together with *relevel()*, for testing for significant differences between pairs of experimental treatments (R script here).

As a reminder, linear models can always be written in the form:

Y

_{i}= μ + A_{i}+ B_{i}+ C_{i}+ … + A_{i}*B_{i}+ A_{i}*C_{i}+ B_{i}*C_{i}+ A_{i}*B_{i}*C_{i}+ … + ɛ_{i}

Where:

- μ is the overall mean of all the Y
_{i} - A
_{i}, B_{i}, and C_{i }are the differences between Y_{i}and the overall μ due to the main effects of A, B, and C - A
_{i}*B, etc are the differences between Y_{i}and the overall μ due to interactions among explanatory variables - ɛ
_{i}is the difference between Y_{i}and μ left unexplained by the model.

A linear model can be thought as the mathematical transcription of our research question. What is the effect of factors A, B, and C on our response variable? Does the effect of each factor on Y depend on other factors? We answer these questions by testing if models including all factors and all their interactions explain significantly more variability in Y than simpler models (including less factors and/or less interactions) do.

In general, the golden steps are:

- have a clear question
- design the study/experiment accordingly
- collect your data
- PLOT THE DATA and start doing qualitative interpretation of the study results
- test your initial question(s) by defining the corresponding linear model in a statistical software (e.g. R)
- assess if the model assumptions are respected (*)
- select the most parsimonious model using anova()
- evaluate differences among treatments using summary()

**Example 1. One-way ANOVA**

The question: do chick weight differ if they are fed differently?

```
# Load data:
data(chickwts) # chickwts is one of the many data sets built
# in the R software by default.
# data() is a function specific for calling these datasets.
# PLOT THE DATA:
boxplot(weight ~ feed,
data = chickwts,
ylab="Weight (grams)")
```

**It seems that there are indeed differences among treatments. Let’s check this statistically:**

```
chick1 <- lm(weight ~ feed, data = chickwts)
par(mfrow=c(2,2)) # prepare a window with room for 4 plots
plot(chick1) # test the assumptions graphically.
```

To know whether the feeding treatment is relevant for predicting chick weight, we must compare this model with the simpler one:

```
chick0 <- lm(weight ~ 1, data = chickwts)
# in this case, this is also the simplest model possible.
```

Which model is more parsimonious?

```
anova(chick1, chick0)
```*Analysis of Variance Table*
*Model 1: weight ~ feed*
*Model 2: weight ~ 1*
* Res.Df RSS Df Sum of Sq F Pr(>F) *
*1 65 195556 *
*2 70 426685 -5 -231129 15.365 5.936e-10 ****
*---*
*Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1*

This test checks if the more complex model explains significantly more variability than the simpler one. In this case the answer is yes, that is, adding information about the food provided to chicks allows more precise estimates of their body weight.

Now, how to estimate whether pairs of specific treatments differ significantly? R shows it in the model’s summary table:

```
summary(chick1)
```*Call:*
*lm(formula = weight ~ feed, data = chickwts)*
*Residuals:*
* Min 1Q Median 3Q Max *
*-123.909 -34.413 1.571 38.170 103.091 *
*Coefficients:*
* Estimate Std. Error t value Pr(>|t|) *
*(Intercept) 323.583 15.834 20.436 < 2e-16 ****
*feedhorsebean -163.383 23.485 -6.957 2.07e-09 ****
*feedlinseed -104.833 22.393 -4.682 1.49e-05 ****
*feedmeatmeal -46.674 22.896 -2.039 0.045567 * *
*feedsoybean -77.155 21.578 -3.576 0.000665 ****
*feedsunflower 5.333 22.393 0.238 0.812495 *
*---*
*Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1*
*Residual standard error: 54.85 on 65 degrees of freedom*
*Multiple R-squared: 0.5417, Adjusted R-squared: 0.5064 *
*F-statistic: 15.36 on 5 and 65 DF, p-value: 5.936e-10*

The treatment levels are ordered alpha-numerically, so that (intercept) refers to the mean weight of chicks fed on casein: 323.583 grams (SE=15.834). The estimate is significantly different from zero (look at the p-value). Estimates for all other levels are given as differences from the reference level. For example, the mean weight of chicks fed on horsebean is (323.583 – 163.383) grams. The p-value shows that the two averages differ significantly.

Now, what if we were interested in the difference in weight of chicks fed on meatmeal versus soybean? The mean weights are (323.583 – 46.674) grams and (323.583 – 77.155) grams respectively, but are they significantly different from each other? The p- values provided refer to their difference from the reference level, so they cannot help us. The trick is to use function *relevel()*:

chickwts$feed <- relevel(chickwts$feed, ref = "meatmeal") # this does NOT change any data, it just tells R # to consider the specified treatment as reference level in the summary. # IMPORTANT! Re-run the linear model: chick1 <- lm(weight ~ feed, data = chickwts) summary(chick1)Call:lm(formula = weight ~ feed, data = chickwts)Residuals:Min 1Q Median 3Q Max-123.909 -34.413 1.571 38.170 103.091Coefficients:Estimate Std. Error t value Pr(>|t|)(Intercept) 276.91 16.54 16.744 < 2e-16 ***feedcasein 46.67 22.90 2.039 0.0456 *feedhorsebean -116.71 23.97 -4.870 7.48e-06 ***feedlinseed -58.16 22.90 -2.540 0.0135 *feedsoybean -30.48 22.10 -1.379 0.1726feedsunflower 52.01 22.90 2.271 0.0264 *---Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1Residual standard error: 54.85 on 65 degrees of freedomMultiple R-squared: 0.5417, Adjusted R-squared: 0.5064F-statistic: 15.36 on 5 and 65 DF, p-value: 5.936e-10

The two levels do not differ significantly, so the feeding regimes “meatmeal” and “soybean” produce chicks with comparable weights. This releveling process can be repeated untill all (relevant) pairwise comparisons have been performed.

**Example 2. Two-way ANOVA**

The same ideas discussed for a linear model with only one explanatory variable can be extended to linear models with two or more explanatory variables.

Let’s say we want to know whether the effect of food on chick weight differ among chicks of different breeds. We would have to run a new experiment, and then analyze the data as follows. First, run the three models we would like to compare:

```
chick_interaction <- lm(weight ~ feed * breed, data = FoodBreedExpt)
chick_additive <- lm(weight ~ feed + breed, data = FoodBreedExpt)
chick_feedonly <- lm(weight ~ feed, data = FoodBreedExpt)
```

* [note: FoodBreedExpt is an imaginary dataset. These data are not provided]*

Then, compare the models using anova():

`anova(chick_interaction, chick_additive)`

If model “chick_interaction” is significantly better than “chick_additive”, it means that chicks from different breeds respond to different feeding regimes differently. If “chick_additive” is more parsimonious, then we should check it against chick_feedonly:

`anova(chick_additive, chick_feedonly)`

If “chick_additive” still “wins”, that means that chicks from different breeds show different body weights overall, but the *differences* in chick weight among feeding treatments are similar regardless of their breed.

Once the most parsimonious model is selected, then we can proceed using *summary() *and* relevel()* to look at differences between pairs of treatments into detail. The procedure is the same shown for Example 1 in the R script. See also this article.

I hope these notes helped. There are other ways to accomplish the result shown above. For example, some people prefer to use *aov()* to perform Analysis of Variance and *TukeyHSD()* for pairwise contrasts. The procedure I described is fairly general (works for regression, ANOVA, ANCOVA and mixed effect models in the same way) and it works pretty well for me. Note that I am not a statistician, so I invite to check the information shown above on a text book if something I wrote sounds weird (or drop me an email). There is plenty of good stats books out there. Among introductory books for R, I particularly like Beckerman and Petchey’s “Getting Started with R” (guess why…).